Glucosylceramides of pig epidermis: structure determination.
نویسندگان
چکیده
Six series of glucosylceramides from pig epidermis have been identified, and their structures have been determined. The structural types identified are: 1, N-acylglucosylsphingosines (33%); 2, N-acylglucosylphytosphingosines (13%); 3, N-(omega-hydroxyacyl)-glucosylsphingosines (3%); 4, N-(alpha-hydroxyacyl)-glucosylphingosines (15%); 5, N-(alpha-hydroxyacyl)-glucosylsphingosines (16%); 6, N-(alpha-hydroxyacyl)-glucosylphytosphingosines (20%). The 4th and 5th classes of glucosylceramides differ in that the former contains mostly 24- to 28-carbon alpha-hydroxyacids, while the latter contains mostly alpha-hydroxypalmitic acid.
منابع مشابه
Acylglucosylceramides of pig epidermis: structure determination.
The acylglucosylceramides of pig epidermis comprise 56% of the total glucosylceramides and 3.3% of the total epidermal lipid by weight. Contrary to an earlier report on this lipid, the amide-linked fatty acid is not a dihydroxypentatriacontadienoic acid; rather, the fatty acids are a series of long chain omega-hydroxy acids consisting of 58% saturates, 35% monoenes, and 7% dienes, with predomin...
متن کاملLipid compositions of cells isolated from pig, human, and rat epidermis.
Epidermal slices from pig, human, and rat skin were treated with dilute buffered trypsin solution (0.005%, w/v), and suspensions of mixed basal and spinous cells were obtained in good yield. Total lipids accounted for approximately 8% of the pig, 10% of the human, and 20% of the rat epidermal cell (dry weight). Phospholipids in pig, human, and rat cells accounted for, respectively, 62%, 53%, an...
متن کاملSphingolipid metabolism during epidermal barrier development in mice.
In rodents, a competent skin barrier to water loss is formed within 2 or 3 days prior to birth. Acquisition of barrier function during rat gestation correlates with the formation of a stratum corneum enriched in ceramides, cholesterol, and fatty acids (Aszterbaum, M., G. K. Menon, K. R. Feingold, and M. L. Williams. 1992. Ontogeny of the epidermal barrier to water loss in the rat: correlation o...
متن کاملLinoleate-rich acylglucosylceramides of pig epidermis: structure determination by proton magnetic resonance.
The structure of the linoleate-rich acylglycosylceramides isolated from pig epidermis has been reinvestigated. Gas-liquid chromatographic analysis of the alditol acetates produced from the sugar component indicated that 90% of the hexose is glucose while the remaining 10% is galactose. The predominance of the beta-D-glucosyl group was confirmed by 360 MHz proton magnetic resonance spectroscopy....
متن کاملDistribution and metabolism of sphingosine in skin after oral administration to mice.
In this study, (3)H- or (13)C(2),D(2)-sphingosine (SPH) was orally administered to mice to assess absorption, mass balance, tissue distribution, and metabolites in the skin. The blood concentration of (3)H-SPH showed a Tmax of 10.7 hr. The radioactivity in the skin reached 763.4 ng eq./g tissue at 12 hr, and decreased to 181.7 ng eq./g tissue at 168 hr. The concentration of radioactivity at 12 ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of lipid research
دوره 24 9 شماره
صفحات -
تاریخ انتشار 1983